Journal: bioRxiv
Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
doi: 10.64898/2026.04.18.719423
Figure Lengend Snippet: (a) SWATH data-based comparison of mechanism protein expressions among mucoepidermoid carcinoma and adenoid cystic carcinoma of salivary gland cancer cells in 2D and 3D cancer immune coculture model. Heat map to visualize hierarchical clustering of mechanism proteins in A-253 and ACC cells in 3D immune co-culture model made with heatmap (2) [gplots R package]. The protein intensities are log10 transformed and are displayed as colours ranging from blue to red as shown in the key. Both rows and columns are clustered using correlation distance and average linkage; Hematoxylin-eosin stained FFPE sample of (b) pleomorphic adenoma of salivary gland; Scale 200 µm; (c) mucoepidermoid carcinoma of salivary gland; Scale 200 µm; (d) adenoid cystic carcinoma of salivary gland; Scale 100 µm; (e) normal parotid salivary gland; Scale 200 µm; (f) bar chart showing ANXA3 relative protein expressions in FFPE samples.
Article Snippet: Four salivary gland condition FFPE slides were used during the project. (i) Adenoid cystic carcinoma of salivary gland (NBP2-30299, Novus Biologicals), Layout: 1X1, Diameter: 4, Thickness: 5 μm, Age: 51 years, Sex: F, Organ: Parotid gland, Pathology: Adenoid Cystic Carcinoma, Tissue Status: Tumor/Cancer, Species: Human; (ii) normal parotid salivary gland (NBP2-30192, Novus Biologicals), Layout: 1X1, Diameter: 4, Thickness: 5 μm, Age: 37 years, Sex: F, Organ: Parotid gland, Tissue Status: Normal, Species: Human; (iii) mucoepidermoid carcinoma of salivary gland (NBP2-30300, Novus Biologicals), Layout: 1X1, Diameter: 4, Thickness: 5 μm, Age: 31 years, Sex: F, Organ: Parotid gland, Pathology: Mucoepidermoid Carcinoma, Tissue Status: Tumor/Cancer, Species: Human; and (iv) pleomorphic adenoma of salivary gland (NBP2-77923, Novus Biologicals), Layout: 1X1, Diameter: 4, Thickness: 5 μm, Age: 63 years, Sex: M, Organ: Parotid gland, Pathology: pleomorphic adenoma, Tissue Status: Tumor, Species: Human.
Techniques: Data-independent acquisition, Comparison, Co-Culture Assay, Transformation Assay, Staining